Microbially induced calcite precipitation (MICP) is a sustainable technology which utilizes biochemical processes, to produce calcium carbonate (CaCO 3 ) in the presence of Urea, calcium chloride (CaCl 2 ), and microorganisms. The application of the MICP process, via biostimulation of natural microorganisms, to a natural sandy soil that was contaminated in the laboratory with hexavalent chromium (Cr 6+ ) and cadmium (Cd 2+ ) was investigated. The improvement of the soil’s geomechanical strength and the increase in its capacity to immobilize both contaminants were evaluated. An experimental program was carried out with bioreactors to evaluate the influence of three main parameters: Cr 6+ (X1), Cd 2+ (X2) and the volume of biocementation solution (X3). The experiment was carried out in the laboratory, with bioreactors, with 5 cm of contaminated soil, 25 cm of uncontaminated soil and 10 cm for insertion of the biocementation solution. Monitoring of urease behavior indicated that initial concentrations of 20 mg.kg −1 and 40 mg.kg −1 for Cr 6+ and Cd 2+ , respectively, were not toxic to urea hydrolysis. Movement of contaminants occurred during the leaching of the biocementation solution, however, contaminant immobilization showed maximum values of 95.67% for Cd 2+ and 87% for Cr 6+ . The uniaxial compressive strength (UCS) test reached values between 114.84 and 917.43 kPa. This indicates that CaCO 3 precipitation occurs even in a toxic environment, immobilizing and promoting the biomineralization of contaminants. In conclusion, MICP could be a promising sustainable technique for application in contaminated soil.
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